Voluntary ex ante transparency notice super-resolution microscope for 3-dimensional functional imaging (3D-SR-FI)

WUR needs a contract for the delivery of a microscope system, that is capable of 3D imaging of molecular and cellular functioning in living tissues, with an optical resolution below the diffraction limit. The general aim of node 2 of the NL-bioimaging consortium is to apply 3D functional imaging in …

CPV: 38515200 Microscopios de fluorescencia, 38510000 Microscopios
Lugar de ejecución:
Voluntary ex ante transparency notice super-resolution microscope for 3-dimensional functional imaging (3D-SR-FI)
Organismo adjudicador:
Wageningen University & Research
Número de premio:
T108298 Voluntary ex ante transparency notice super-resolution microscope for 3-dimensional functional imaging (3D-SR-FI)

1. Buyer

1.1 Buyer

Official name : Wageningen University & Research

2. Procedure

2.1 Procedure

Title : Voluntary ex ante transparency notice super-resolution microscope for 3-dimensional functional imaging (3D-SR-FI)
Description : WUR needs a contract for the delivery of a microscope system, that is capable of 3D imaging of molecular and cellular functioning in living tissues, with an optical resolution below the diffraction limit. The general aim of node 2 of the NL-bioimaging consortium is to apply 3D functional imaging in complex living-cellular systems. 3D imaging of multicellular organisms like organoids or plants allows to obtain the landscape of molecular events and to reveal the mode of action of proteins in their natural habitat. Specifically at WUR, the focus of the 3D functional imaging node will be on ‘high resolution’, ‘endogenous expression’ and ‘FLIM/FCS’. The main requirement for the new system is that specimen can be imaged with a resolution beyond the diffraction limit (super-resolution microscopy or optical nanoscopy). The system should be able to image fluorescent proteins (FPs) expressed at endogenous levels in living plant tissues. Furthermore, the system should have functional imaging tools, such as fluorescence lifetime imaging microscopy (FLIM). Moreover, the conditions for super-resolution should not interfere with (multi-)cellular functioning. The main challenge for the system is to suppress autofluorescence in these samples. Fluorescence background from e.g. chloroplasts in living plant cells or tissues is typically much stronger than the signals from the fluorescent proteins of interest. The detection part of the microscope should therefore be able to deal with a high number of photons, and it should be able to distinguish weak signals of interest from strong background fluorescence. Proven ways how this can be achieved are tuneability of excitation and emission wavelength (white light laser, tuneable filters, spectral unmixing) and/or time gating with photon counting detectors. The second challenge is to go from 2D to 3D functional imaging. This means that more spatial information must be obtained within the same – sample and experiment dependent – time window than before. We therefore seek a 3D FLIM system that is fast allowing real time 3D visualization of the lifetime information (or simply lifetime contrast). This is prioritized over high time resolution FLIM that requires post processing of the data. When experiments require more detailed information, FLIM and fluorescence correlation spectroscopy (FCS) with high temporal resolution and accuracy can be performed (in 2D) on the currently available laser scanning microscopes within the MSC.
Procedure identifier : 2521c827-75b9-402a-b5f4-66b3a1075109
Internal identifier : T108298 Voluntary ex ante transparency notice super-resolution microscope for 3-dimensional functional imaging (3D-SR-FI)
Type of procedure : Negotiated without prior call for competition

2.1.1 Purpose

Main nature of the contract : Supplies
Main classification ( cpv ): 38515200 Fluorescent microscopes
Additional classification ( cpv ): 38510000 Microscopes

2.1.2 Place of performance

Country : Netherlands
Anywhere in the given country
Additional information : See documentation

2.1.4 General information

Legal basis :
Directive 2014/24/EU

5. Lot

5.1 Lot technical ID : LOT-0000

Title : Voluntary ex ante transparency notice super-resolution microscope for 3-dimensional functional imaging (3D-SR-FI)
Description : WUR needs a contract for the delivery of a microscope system, that is capable of 3D imaging of molecular and cellular functioning in living tissues, with an optical resolution below the diffraction limit. The general aim of node 2 of the NL-bioimaging consortium is to apply 3D functional imaging in complex living-cellular systems. 3D imaging of multicellular organisms like organoids or plants allows to obtain the landscape of molecular events and to reveal the mode of action of proteins in their natural habitat. Specifically at WUR, the focus of the 3D functional imaging node will be on ‘high resolution’, ‘endogenous expression’ and ‘FLIM/FCS’. The main requirement for the new system is that specimen can be imaged with a resolution beyond the diffraction limit (super-resolution microscopy or optical nanoscopy). The system should be able to image fluorescent proteins (FPs) expressed at endogenous levels in living plant tissues. Furthermore, the system should have functional imaging tools, such as fluorescence lifetime imaging microscopy (FLIM). Moreover, the conditions for super-resolution should not interfere with (multi-)cellular functioning. The main challenge for the system is to suppress autofluorescence in these samples. Fluorescence background from e.g. chloroplasts in living plant cells or tissues is typically much stronger than the signals from the fluorescent proteins of interest. The detection part of the microscope should therefore be able to deal with a high number of photons, and it should be able to distinguish weak signals of interest from strong background fluorescence. Proven ways how this can be achieved are tuneability of excitation and emission wavelength (white light laser, tuneable filters, spectral unmixing) and/or time gating with photon counting detectors. The second challenge is to go from 2D to 3D functional imaging. This means that more spatial information must be obtained within the same – sample and experiment dependent – time window than before. We therefore seek a 3D FLIM system that is fast allowing real time 3D visualization of the lifetime information (or simply lifetime contrast). This is prioritized over high time resolution FLIM that requires post processing of the data. When experiments require more detailed information, FLIM and fluorescence correlation spectroscopy (FCS) with high temporal resolution and accuracy can be performed (in 2D) on the currently available laser scanning microscopes within the MSC.
Internal identifier : T108298 Voluntary ex ante transparency notice super-resolution microscope for 3-dimensional functional imaging (3D-SR-FI)

5.1.1 Purpose

Main nature of the contract : Supplies
Main classification ( cpv ): 38515200 Fluorescent microscopes
Additional classification ( cpv ): 38510000 Microscopes

5.1.2 Place of performance

Country : Netherlands
Anywhere in the given country
Additional information : See documentation

5.1.6 General information

Procurement Project not financed with EU Funds.
The procurement is covered by the Government Procurement Agreement (GPA) : yes

5.1.16 Further information, mediation and review

Review organisation : Rechtbank Gelderland

6. Results

Value of all contracts awarded in this notice : 800 000 Euro

6.1 Result lot ldentifier : LOT-0000

6.1.2 Information about winners

Winner :
Official name : Leica Microsystems B.V.
Tender :
Tender identifier : T108298
Identifier of lot or group of lots : LOT-0000
The tender was ranked : yes
Subcontracting : No
Subcontracting value is known : no
Subcontracting percentage is known : no
Contract information :
Identifier of the contract : T108298
Title : Turnkey delivery including maintenance super-resolution microscope for 3-dimensional functional imaging (3D-SR-FI)
Date on which the winner was chosen : 12/09/2024
The contract is awarded within a framework agreement : no

8. Organisations

8.1 ORG-0001

Official name : Wageningen University & Research
Registration number : 09215846
Postal address : Droevendaalsesteeg 4
Town : Wageningen
Postcode : 6708PB
Country subdivision (NUTS) : Zuidwest-Gelderland ( NL224 )
Country : Netherlands
Contact point : Jasper van Kasteren
Telephone : +10317488962
Internet address : https://www.wur.nl
Roles of this organisation :
Buyer

8.1 ORG-0002

Official name : Rechtbank Gelderland
Registration number : 82940827
Department : locatie Arnhem
Postal address : Walburgstraat 2-4
Town : Arnhem
Postcode : 6811CD
Country subdivision (NUTS) : Arnhem/Nijmegen ( NL226 )
Country : Netherlands
Telephone : +31 0883612000
Roles of this organisation :
Review organisation

8.1 ORG-0003

Official name : Leica Microsystems B.V.
Size of the economic operator : Large
The organisation is a natural person
Registration number : 27082602
Postal address : Vlierweg 20
Town : Amsterdam
Postcode : 1032 LG
Country : Netherlands
Telephone : 0704132100
Roles of this organisation :
Tenderer
Winner of these lots : LOT-0000
The winner is listed on a regulated market

11. Notice information

11.1 Notice information

Notice identifier/version : 2e47a30e-91c5-4654-82a6-c29b78ebdc38 - 01
Form type : Direct award preannouncement
Notice type : Voluntary ex-ante transparency notice
Notice dispatch date : 13/09/2024 14:59 +00:00
Notice dispatch date (eSender) : 13/09/2024 15:00 +00:00
Languages in which this notice is officially available : English

11.2 Publication information

Notice publication number : 00558452-2024
OJ S issue number : 181/2024
Publication date : 17/09/2024