Multiphoton imaging system

The research group of Stefanos Stagkourakis will carry out a project focused on all-optical investigations that provide the capability of imaging and manipulating neuronal activity at the same time. Specifically, multiphoton imaging of Ca2+ and voltage indicators will be used to study neuron activity on large neuron populations in vivo …

CPV: 38510000 Microscopios, 38515200 Microscopios de fluorescencia
Lugar de ejecución:
Multiphoton imaging system
Organismo adjudicador:
Karolinska Institutet
Número de premio:
2-4528/2024

1. Buyer

1.1 Buyer

Official name : Karolinska Institutet

2. Procedure

2.1 Procedure

Title : Multiphoton imaging system
Description : The research group of Stefanos Stagkourakis will carry out a project focused on all-optical investigations that provide the capability of imaging and manipulating neuronal activity at the same time. Specifically, multiphoton imaging of Ca2+ and voltage indicators will be used to study neuron activity on large neuron populations in vivo and in vitro. Successful pursuit of this work requires simultaneous recording of activity and manipulation through multiphoton photostimulation of pre-selected neuron ensembles to study a causal role of specific neuron subpopulations in behavior. A key component of the project is complementing the head-fixed in vivo neural activity and ensemble manipulation recordings with neuron activity imaging in freely moving animals through the Inscopix miniscope technology. In order to be able to carry out the research, KI needs to purchase an Multiphoton imaging system that meets the following requirements: a. The system must have a volumetric imaging solution which is decoupled from the photostimulation pathway while still offering a travel range that will allow imaging between planes of interest that are spatially separated by at least 390um in Z. This is needed to allow imaging and interrogation of the areas of interest across layers. Without that level of travel, simultaneous imaging and stimulation at multiple planes of interest would not possible where they are needed. Decoupling the photostimulation and imaging beams is necessary otherwise biological temporal resolution will not be observed due to the delay between stimulating, refocusing and imaging. b. The system must have a Spatial Light Modulator (SLM) to produce an axial resolution no larger than 10um. This is necessary to ensure that only the cells of interest are stimulated. Stimulation of multiple cells throughout a 3D volume is a key part of the project. If the axial resolution is larger than 10um, there is a risk that multiple cell layers can be stimulated instead of it being limited to the areas of interest c. It’s important that the objective of the system can be rotated, without sacrificing the SLM resolution, excitation, or collection efficiency. This is necessary when undertaking behavioral experiments as tilting the animal can cause it to behave unnaturally, a variable which is difficult to account for and one which can compromise the scientific integrity of the experiment itself. Maintaining this integrity should not come at the expense of signal otherwise power would need to be increased, causing unnecessary distress to the animal and a further experimental variable. d. The system must have detectors that are physically protected from any transmitted lightused for the imaging of brain slices. There must be a protection circuit in place that willnot allow any multiphoton detection to occur while the transmitted light source isactive. e. The research involves correlative studies between head-fixed and freely moving mouse models. Having access to a cloud-based software platform that can incorporate both data types is necessary as it enables faster, more efficient batch processing of computationally intense features. f. The system must be able to image and stimulate independently throughout the whole volume of a brain slice (up to 300µm depth) with a switching speed between planes of around 15ms. This speed and depth combination is especially valuable for capturing dynamic processes in 3D and for stimulating specific preselected brain populations with temporal precision. To KI’s knowledge there is no other supplier that offers a setup with proven equivalent functions. KI will sign a contract with Bruker after a standstill period of 10 days after publishing this Voluntary ex ante transparency notice.
Procedure identifier : 829cdc2e-e836-42d5-93d7-0ec14c7cfdb0
Internal identifier : 2-4528/2024
Type of procedure : Negotiated without prior call for competition

2.1.1 Purpose

Main nature of the contract : Supplies
Main classification ( cpv ): 38510000 Microscopes
Additional classification ( cpv ): 38515200 Fluorescent microscopes

2.1.2 Place of performance

Anywhere

2.1.4 General information

Legal basis :
Directive 2014/24/EU
6 kap 14 § 2 p. LOU -

5. Lot

5.1 Lot technical ID : LOT-0000

Title : Multiphoton imaging system
Description : The research group of Stefanos Stagkourakis will carry out a project focused on all-optical investigations that provide the capability of imaging and manipulating neuronal activity at the same time. Specifically, multiphoton imaging of Ca2+ and voltage indicators will be used to study neuron activity on large neuron populations in vivo and in vitro. Successful pursuit of this work requires simultaneous recording of activity and manipulation through multiphoton photostimulation of pre-selected neuron ensembles to study a causal role of specific neuron subpopulations in behavior. A key component of the project is complementing the head-fixed in vivo neural activity and ensemble manipulation recordings with neuron activity imaging in freely moving animals through the Inscopix miniscope technology. In order to be able to carry out the research, KI needs to purchase an Multiphoton imaging system that meets the following requirements: a. The system must have a volumetric imaging solution which is decoupled from the photostimulation pathway while still offering a travel range that will allow imaging between planes of interest that are spatially separated by at least 390um in Z. This is needed to allow imaging and interrogation of the areas of interest across layers. Without that level of travel, simultaneous imaging and stimulation at multiple planes of interest would not possible where they are needed. Decoupling the photostimulation and imaging beams is necessary otherwise biological temporal resolution will not be observed due to the delay between stimulating, refocusing and imaging. b. The system must have a Spatial Light Modulator (SLM) to produce an axial resolution no larger than 10um. This is necessary to ensure that only the cells of interest are stimulated. Stimulation of multiple cells throughout a 3D volume is a key part of the project. If the axial resolution is larger than 10um, there is a risk that multiple cell layers can be stimulated instead of it being limited to the areas of interest c. It’s important that the objective of the system can be rotated, without sacrificing the SLM resolution, excitation, or collection efficiency. This is necessary when undertaking behavioral experiments as tilting the animal can cause it to behave unnaturally, a variable which is difficult to account for and one which can compromise the scientific integrity of the experiment itself. Maintaining this integrity should not come at the expense of signal otherwise power would need to be increased, causing unnecessary distress to the animal and a further experimental variable. d. The system must have detectors that are physically protected from any transmitted lightused for the imaging of brain slices. There must be a protection circuit in place that willnot allow any multiphoton detection to occur while the transmitted light source isactive. e. The research involves correlative studies between head-fixed and freely moving mouse models. Having access to a cloud-based software platform that can incorporate both data types is necessary as it enables faster, more efficient batch processing of computationally intense features. f. The system must be able to image and stimulate independently throughout the whole volume of a brain slice (up to 300µm depth) with a switching speed between planes of around 15ms. This speed and depth combination is especially valuable for capturing dynamic processes in 3D and for stimulating specific preselected brain populations with temporal precision. To KI’s knowledge there is no other supplier that offers a setup with proven equivalent functions. KI will sign a contract with Bruker after a standstill period of 10 days after publishing this Voluntary ex ante transparency notice.
Internal identifier : 2-4528/2024

5.1.1 Purpose

Main nature of the contract : Supplies
Main classification ( cpv ): 38510000 Microscopes
Additional classification ( cpv ): 38515200 Fluorescent microscopes

5.1.2 Place of performance

Anywhere
Additional information :

5.1.6 General information

Procurement Project not financed with EU Funds.
The procurement is covered by the Government Procurement Agreement (GPA) : no

5.1.16 Further information, mediation and review

Review organisation : Förvaltningsrätten
TED eSender : Mercell Holding ASA

6. Results

Direct award :
Justification for direct award : The contract can be provided only by a particular economic operator because of an absence of competition for technical reasons
Other justification :

6.1 Result lot ldentifier : LOT-0000

6.1.2 Information about winners

Winner :
Official name : Bruker France S.A.S
Tender :
Tender identifier : 2-4528/2024
Identifier of lot or group of lots : LOT-0000
Value of the result : 700 000 Euro
Subcontracting value is known : no
Subcontracting percentage is known : no
Contract information :
Identifier of the contract : 2-4528/2024
Title : Multiphoton imaging system
Date on which the winner was chosen : 05/12/2024
The contract is awarded within a framework agreement : no

8. Organisations

8.1 ORG-0001

Official name : Karolinska Institutet
Registration number : 2021002973
Department : Upphandlare
Postal address : Nobels väg 5
Town : STOCKHOLM
Postcode : 17177
Country : Sweden
Contact point : Hanna Glad
Telephone : +46 08-52480000
Internet address : http://www.ki.se
Roles of this organisation :
Buyer

8.1 ORG-0002

Official name : Förvaltningsrätten
Registration number : 202100-2742
Town : Stockholm
Postcode : 115 76
Country subdivision (NUTS) : Stockholms län ( SE110 )
Country : Sweden
Contact point : Förvaltningsrätten i Stockholm
Telephone : 08-561 680 00
Roles of this organisation :
Review organisation

8.1 ORG-0003

Official name : Bruker France S.A.S
The organisation is a natural person
Registration number : 311 020 911 00013
Department : Sale
Town : Wissembourg
Country : France
Contact point : Pascale Nicolas/Fabienne Gandrillo
Telephone : +33 1 72 86 61 00
Internet address : https://www.bruker.com
Roles of this organisation :
Tenderer
Winner of these lots : LOT-0000

8.1 ORG-0004

Official name : Mercell Holding ASA
Registration number : 980921565
Postal address : Askekroken 11
Town : Oslo
Postcode : 0277
Country subdivision (NUTS) : Oslo ( NO081 )
Country : Norway
Contact point : eSender
Telephone : +47 21018800
Fax : +47 21018801
Internet address : http://mercell.com/
Roles of this organisation :
TED eSender

11. Notice information

11.1 Notice information

Notice identifier/version : bf72f5ad-7d3d-49bf-ba18-330c8f3f3350 - 01
Form type : Direct award preannouncement
Notice type : Voluntary ex-ante transparency notice
Notice dispatch date : 06/12/2024 15:01 +00:00
Notice dispatch date (eSender) : 06/12/2024 15:01 +00:00
Languages in which this notice is officially available : English

11.2 Publication information

Notice publication number : 00754171-2024
OJ S issue number : 240/2024
Publication date : 10/12/2024